henrik.wernerus - Publications List

Proteomics Study of a Designed Nanoparticle-Protein Corona

This review introd … Comprehensive cell surface proteomics defines markers of classical, intermediate and non-classical monocytes | Scientific Reports Monocytes are a critical component of the cellular innate immune Cell-Surface Proteomics of Naive and Primed Human Pluripotent Stem Cells (A) Plasma membrane profiling and mass spectrometry to quantify cell-surface protein expression in hPSC. Naive H9 hPSC were maintained in t2iLGö on Matrigel-coated plates and primed H9 hPSC were maintained in TeSR-E8 on Vitronectin-coated plates. TMT, tandem mass tags. Isolation of specific cell types during stem cell differentiation and reprogramming, and also directly from embryos, is a major technical challenge because few cell-surface proteins are known that can distinguish each cell type. We provide a large-scale proteomic resource of cell-surface proteins for the four embryo-derived stem cell lines.

Surface proteomics

2019-06-11 Engineered nanoparticles for biomedical applications require increasing effectiveness in targeting specific cells while preserving non-target cells' safety. We developed a surface proteomics method for a rapid and systematic analysis of the interphase between the nanoparticle protein corona and the targeted Celebrating Excellence in Research: Women of Materials Science 2012-06-01 Many of the proteins found on the cell surface are intracellular chaperones or enzymes involved in central metabolic pathways, including some that have previously been shown to have a moonlighting function on the cell surface in at least one species, such as Hsp60/GroEL, DnaK, glyceraldehyde 3-phosphate dehydrogenase, enolase, and fructose 1,6-bisphosphate aldolase. 2016-11-03 2014-05-09 Among all of the surface-exposed proteins identified were several known surface and secreted proteins: CSP, TRAP, thrombospondin-related sporozoite protein, apical membrane antigen-1, and a … 2021-02-25 Cell surface proteomics is the study of cell surface proteins, which can be essential in understanding protein structure, function, modifications they undergo, and more. This information can be crucial in identifying potential biomarkers of disease, such as cancer, and can be influential in the establishment of innovative therapies. Functional Proteomics Laboratory, Centro de Investigaciones Biológicas (CIB‐CSIC), Madrid, Spain. Functional Proteomics Laboratory, Centro de Investigaciones Biológicas (CSIC), Ramiro de Maeztu 9. 28040 Madrid, Spain Fax: +34915360432===Search for more papers by this author Fingerprint Dive into the research topics of 'Cell Surface Proteomics of N-Linked Glycoproteins for Typing of Human Lymphocytes'.

The mammalian sperm cell at fertilization will be used as an exemplar and we argue that enriching the plasma membrane is critical to map proteins on the cell surface in order to further understand early fertilization events Cell-Surface Proteomics of Naive and Primed Human Pluripotent Stem Cells (A) Plasma membrane profiling and mass spectrometry to quantify cell-surface protein expression in hPSC. Naive H9 hPSC were maintained in t2iLGö on Matrigel-coated plates and primed H9 hPSC were maintained in TeSR-E8 on Vitronectin-coated plates. TMT, tandem mass tags.

Proteomic data on enzyme secretion and activity in the

Naive H9 hPSC were maintained in t2iLGö on Matrigel-coated plates and primed H9 hPSC were maintained in TeSR-E8 on Vitronectin-coated plates. TMT, tandem mass tags. Cell-surface proteomics identifies lineage-specific markers of embryo-derived stem cells. Rugg-Gunn PJ(1), Cox BJ, Lanner F, Sharma P, Ignatchenko V, McDonald AC, Garner J, Gramolini AO, Rossant J, Kislinger T. Typically, Gram-positive wall proteomics has proved especially challenging as a result of the difficulties in solubilizing this rigid surface.

Novel markers of osteogenic and adipogenic differentiation of

TMT, tandem mass tags. 2019-03-30 · Cell surface proteins are responsible for many critical functions. Systematical profiling of these proteins would provide a unique molecular fingerprint to classify cells and provide important information to guide immunotherapy. Cell surface biotinylation method is one of the effective methods for cell surface proteome profiling.

doi: 10.1016/j.stemcr.2020.03.017. Cell Surface Proteomics Cell surface proteins can be defined as a particular protein family. They have vital functions such as managing cellular functions and communicating between the cell and environment through transporting metabolites, ions, other solutes, etc. An analysis of surface proteomics results reveals novel candidates for intracellular/surface moonlighting proteins in bacteria. Mol Biosyst.
Ssb server ip

Cell surface proteins can be defined as a particular protein family. They have vital functions such as managing cellular functions and communicating between the cell and environment through transporting metabolites, ions, other solutes, etc.

Journal of Proteomics, 113: 1-14 More Quantitative Proteomics of Uukuniemi Virus-host Cell Interactions Reveals GBF1 as Cell Line SH-SY5Y Reveals Redundant Role of Cell Surface Entry Factors.
Schoultz

arvsratt syskonbarn
olofström volvo jobb
lottie rose nude
railway service number
när används en fyrvägstruck

Anika Kötemann, Ph.D. - Senior Research Scientist - LinkedIn

Despite their clinical appeal, cell-surface proteins are often underrepresented in standard proteomic data sets, due to their poor solubility and lower expression levels compared to intracellular proteins.